Repository of Research and Investigative Information

Repository of Research and Investigative Information

Larestan University of Medical Sciences

CRISPR-mediated modification of DNA methylation pattern in the new era of cancer therapy

(2020) CRISPR-mediated modification of DNA methylation pattern in the new era of cancer therapy. Epigenomics. pp. 1845-1859. ISSN 1750-1911

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In the last 2 decades, a wide variety of studies have been conducted on epigenetics and its role in various cancers. A major mechanism of epigenetic regulation is DNA methylation, including aberrant DNA methylation variations such as hypermethylation and hypomethylation in the promoters of critical genes, which are commonly detected in tumors and mark the early stages of cancer development. Therefore, epigenetic therapy has been of special importance in the last decade for cancer treatment. In epigenetic therapy, all efforts are made to modulate gene expression to the normal status. Importantly, recent studies have shown that epigenetic therapy is focusing on the new gene editing technology, CRISPR-Cas9. This tool was found to be able to effectively modulate gene expression and alter almost any sequence in the genome of cells, resulting in events such as a change in acetylation, methylation, or histone modifications. Of note, the CRISPR-Cas9 system can be used for the treatment of cancers caused by epigenetic alterations. The CRISPR-Cas9 system has greater advantages than other available methods, including potent activity, easy design and high velocity as well as the ability to target any DNA or RNA site. In this review, we described epigenetic modulators, which can be used in the CRISPR-Cas9 system, as well as their functions in gene expression alterations that lead to cancer initiation and progression. In addition, we surveyed various species of CRISPR-dead Cas9 (dCas9) systems, a mutant version of Cas9 with no endonuclease activity. Such systems are applicable in epigenetic therapy for gene expression modulation through chemical group editing on nucleosomes and chromatin remodeling, which finally return the cell to the normal status and prevent cancer progression.

Item Type: Article
Keywords: cancer therapy CRISPR-Cas9 DNA methylation epigenetics gene editing on-target expression mutations epigenome system demethylation promoter 5-methylcytosine hypomethylation transcription Genetics & Heredity
Divisions: Education Vice-Chancellor Department > Faculty of Nursing and Midwifery > Department of Nursing
Page Range: pp. 1845-1859
Journal or Publication Title: Epigenomics
Journal Index: ISI
Volume: 12
Number: 20
Identification Number:
ISSN: 1750-1911
Depositing User: خانم موهبت شیوخی

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