Repository of Research and Investigative Information

Repository of Research and Investigative Information

Larestan University of Medical Sciences

Formulation of a recombinant HIV-1 polytope candidate vaccine with naloxone/alum mixture: Induction of multi-cytokine responses with a higher regulatory mechanism

(2021) Formulation of a recombinant HIV-1 polytope candidate vaccine with naloxone/alum mixture: Induction of multi-cytokine responses with a higher regulatory mechanism. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. ISSN 1600-0463 (Electronic) 0903-4641 (Linking)

Full text not available from this repository.

Official URL: https://www.ncbi.nlm.nih.gov/pubmed/33539574

Abstract

BACKGROUND: The potency of a vaccine highly depends upon the nature of the adjuvant used. There are a variety of ineffective vaccines, such as HIV-1 vaccine candidates, that need to be optimized with new adjuvant formulations to improve vaccine potency and efficacy. Studies show the potency of naloxone (NLX)/alum mixture in the induction of Th1/Th2 response for vaccine. However, other immunologic patterns inducing by this adjuvant and its immunoregulatory effect is unclear. In this regard, the aim of the present study was to investigate the effect of the NLX/alum mixture, as an adjuvant, on cytokine networks and immunoregulatory activity for an HIV-1 polytope vaccine. METHODS: BALB/c mice were divided into six groups (n=6) and immunized subcutaneously with 10 mug of the vaccine formulated with NLX/alum, NLX, alum, and Freund's adjuvants. At the same time, the mice in the control groups received an equal volume of PBS or NLX. The lymphocyte proliferation assay was carried out using the BrdU method. ELISA was used to measure the levels of IFN-gamma, IL-2, IL-4, IL-10, IL-12, and IL-17 cytokines, total IgG, as well as IgG1 and IgG2a subtypes in serum samples. RESULTS: Our findings showed that mice receiving the NLX/alum-adjuvanted vaccine exhibited increased antibody levels compared with other groups. In addition, there was a considerable difference in the levels of IgG1, IgG2a, IFN-gamma, IL-2, IL-10, IL-12, and IL-17 in mice receiving the NLX/alum-adjuvanted vaccine as compared with other groups. CONCLUSION: The NLX/alum mixture, as an adjuvant, may have a positive effect on the induction of multi-cytokine responses as well as the increased level of IL-10, showing its higher immunogenicity with a higher immunoregulatory mechanism.

Item Type: Article
Keywords: Adjuvant HIV-1 polytope vaccine Immunoregulatory Naloxone (NLX)/Alum mixture Th1/Th2/Th17 Cytokines
Divisions: Education Vice-Chancellor Department > Faculty of Nursing and Midwifery > Department of Nursing
Journal or Publication Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Journal Index: Pubmed
Identification Number: https://doi.org/10.1111/apm.13122
ISSN: 1600-0463 (Electronic) 0903-4641 (Linking)
Depositing User: خانم موهبت شیوخی
URI: http://eprints.larums.ac.ir/id/eprint/343

Actions (login required)

View Item View Item